A data base for PCR primers in the chloroplast genome
Chloroplast DNA primer maps

At this time, primers are presented as primer maps (see further down the page). These are based on the tobacco chloroplast DNA sequence (GenBank CHNTXX), with genes indicated as red blocks, and primers indicated by arrows (I apologize for the quality of these pictures, I am working on improvements). If your connection is unsuitable for looking at the whole page including 16 picture files of 50-100 kb each, try the links to the individual pictures:

Large single copy region (LSC):

part 1: trnI, rpl23 to rps16
part 2: trnQ to atpI
part 3: atpF to trnD
part 4: psbM to psaB
part 5: trnG to trnS
part 6: trnS to atpE
part 7: trnM to accD (includes rbcL)
part 8: accD to rpl33
part 9: rpl33 to rpl20
part 10: rpl20 to petD
part 11: rpoA to ycf2

Inverted repeat B (IRB):

part 11: rpoA to ycf2
part 12: rps12 to 23S rRNA
part 13: 23S rRNA to ycf5 (includes ndhF)

Small single copy region (SSC):

part 13: 23S rRNA to ycf5 (includes ndhF)
part 14: trnL to ndhH
part 15: ndhH to trnN

Inverted repeat A:

part 16: trnR to rRNA 16
part 1: trnI, rpl23 to rps16

 chloroplast map 01
 chloroplast map 02
 chloroplast map 03
 chloroplast map 04
 chloroplast map 05
 chloroplast map 06
 chloroplast map 07
 chloroplast map 08
 chloroplast map 09
 chloroplast map 10
 chloroplast map 11
 part 12
 part 13
 part 14
 part 15
 part 16

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