| A data base for PCR primers in the chloroplast genome | |
| Chloroplast DNA primer maps |
At this time, primers are presented as primer maps (see further down the page). These are based on the tobacco chloroplast DNA sequence (GenBank CHNTXX), with genes indicated as red blocks, and primers indicated by arrows (I apologize for the quality of these pictures, I am working on improvements). If your connection is unsuitable for looking at the whole page including 16 picture files of 50-100 kb each, try the links to the individual pictures:
Large single copy region (LSC):
part 1: trnI, rpl23 to rps16
part 2: trnQ to atpI
part 3: atpF to trnD
part 4: psbM to psaB
part 5: trnG to trnS
part 6: trnS to atpE
part 7: trnM to accD (includes rbcL)
part 8: accD to rpl33
part 9: rpl33 to rpl20
part 10: rpl20 to petD
part 11: rpoA to ycf2
Inverted repeat B (IRB):
part 11: rpoA to ycf2
part 12: rps12 to 23S rRNA
part 13: 23S rRNA to ycf5 (includes ndhF)
Small single copy region (SSC):
part 13: 23S rRNA to ycf5 (includes
ndhF)
part 14: trnL to ndhH
part 15: ndhH to trnN
Inverted repeat A:
part 16: trnR to rRNA 16
part 1: trnI, rpl23 to rps16
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