University of Laval (ULAVAL Québec, Canada)
Organisation and competences: The Center for Forest Research is a multi-institutional organization has well-recognized strengths in forest genetics and genomics in cluding large-scale research projects Arborea (I and II) and the Canada Research Chair in Forest and Environmental Genomics. Activities that are relevant for this proposal include genomics research on spruces (P. glauca, P. marianna, P. rubens, primarily) and pines (P. banksiana, P. monticola). We recently joined forces with the University of British Columbia and submitted a proposal (SMarTForest) to carry out de novo genome sequencing in Picea glauca, as well as develop marker systems to enable the application of marker assisted selection in Canada, from 04/2011 to 03/2014. Key activities include gene discovery and high-throughput sequencing; marker development for tree breeding using multiplexed genotyping methods; population and comparative genomics; quantitative genetics of wood structure; linkage mapping; transcriptome analysis and functional genomics of wood formation. See references.
Role: Direct contributions will be made to WP1, WP3 and WP6 by providing access to results in advance of publication, sharing methods. Particular emphasis will concern WP3 on comparative genomics. We are using high density genetic mapping to position 8000 cDNAs onto the spruce linkage groups by the end of 2011. We can also contribute sequences from BACs each containing a different single copy gene. In WP1, we will share our experience are using sequence capture methods for SNP discovery. If our SMarTForest proposal is funded, we will also be in a position to contribute whole genome sequence data. Additionally, for WP2, transcriptome analysis tools and methods for spruces that could help part of the proposed work on pines.
Staff members' profile:
Dr. John MacKay- is a professor of forest genomics, and lead investigator of the Arborea project and of the SMarTForest research proposal. He will be in charge of contributions to WP1 (genome sequencing), WP2 and WP3 (BAC sequences).
Dr. Jean Bousquet- He is professor at ULaval and the director of the Canada Research Chair in Forest and Environmental Genomics and co-leader of the Arborea project. He will contribute to WP1 (SNP discovery methods) and WP3 (high density genetic mapping). Collaborators. Researchers at Université Laval have developed significant collaborations that enable their research and will contribute to the feasibility of the proposed research.